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mammalian expression vector pires2 egfp  (TaKaRa)


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    TaKaRa mammalian expression vector pires2 egfp
    Mammalian Expression Vector Pires2 Egfp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 422 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pires2+egfp+expression+vector/pIRES2-AcGFP1+Vector/pm40610563-374-13-17
    Average 96 stars, based on 422 article reviews
    mammalian expression vector pires2 egfp - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
    Article Snippet: .. After 48 h, the cells have reached a confluence of approximately 80 to 90% and are ready for transfection. c. Transiently transfect the cells with 1 to 2 μg μL −1 cDNA coding for hTRPC6 (NP_004612) in pIRES2-EGFP expression vector (Clontech) using lipofection with GeneJuice® (Merck Millipore) according to the manufacturer’s instructions. d. 24 to 48 h after transfection, and 1–2 h prior to the loading with PhoDAG or OptoDArG, split the transfected HEK293T cells from one well plate onto 3 to 5 glass-bottom dishes (WPI, FluoroDish FD35-100, diameter of glass bottom 23 mm). .. For equal distribution and separation of the transfected HEK293T cells, wash the cells once with 1 mL Dulbecco’s phosphate buffered saline modified without calcium chloride and magnesium chloride (DPBS, D8537, Sigma-Aldrich) and detach the cells by applying 300 μL Trypsin-EDTA solution (T3924, Sigma-Aldrich) for 20–30 s followed by application of 1.5 mL culture medium.

    Expressing:

    Article Title: A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
    Article Snippet: .. After 48 h, the cells have reached a confluence of approximately 80 to 90% and are ready for transfection. c. Transiently transfect the cells with 1 to 2 μg μL −1 cDNA coding for hTRPC6 (NP_004612) in pIRES2-EGFP expression vector (Clontech) using lipofection with GeneJuice® (Merck Millipore) according to the manufacturer’s instructions. d. 24 to 48 h after transfection, and 1–2 h prior to the loading with PhoDAG or OptoDArG, split the transfected HEK293T cells from one well plate onto 3 to 5 glass-bottom dishes (WPI, FluoroDish FD35-100, diameter of glass bottom 23 mm). .. For equal distribution and separation of the transfected HEK293T cells, wash the cells once with 1 mL Dulbecco’s phosphate buffered saline modified without calcium chloride and magnesium chloride (DPBS, D8537, Sigma-Aldrich) and detach the cells by applying 300 μL Trypsin-EDTA solution (T3924, Sigma-Aldrich) for 20–30 s followed by application of 1.5 mL culture medium.

    Article Title: C/EBPbeta-2 confers EGF-independent growth and disrupts the normal acinar architecture of human mammary epithelial cells
    Article Snippet: .. Next, the His-tagged C/EBPbeta-3 sequences were subcloned into pIRES2-EGFP expression vector (Clontech, Palo Alto, CA). ..

    Article Title: Helix 8 is the essential structural motif of mechanosensitive GPCRs
    Article Snippet: In case of H 1 R-ct-b constructs and of the truncated H 1 R, 0.5 μg G qα -protein , which was a gift from Bruce Conklin, was co-transfected. .. For electrophysiological whole-cell measurements, HEK293 cells were co-transfected with 2 μg human TRPC6 (accession no.: NP_004612) in pIRES2-EGFP expression vector (Clontech) and 0.5 μg H 1 R-mut or CHO-K1 cells were co-transfected with 1 μg rat inward-rectifier potassium channel Kir3.1 (accession no.: NM_031610) and human inward-rectifier potassium channel Kir3.2 (accession no.: NP_002231) in bicistronic pIRES expression vector (Clontech) (Kir3.1-IRES-Kir3.2, ‘Kir3.1/3.2’) and with 0.5 μg human chemokine type 4 receptor (hCXCR4, (accession no.: NM_001295) or human dopamine D2 receptor (hD 2 R, (accession no.: NM_000795) and 0.2 μg cerulean in pcDNA3.1 expression vector (Clontech). .. Site-directed mutagenesis was performed on the guinea pig histamine H receptor in pcDNA3.1( + ) (accession number: BAA03669), on the human GnRH receptor in pcDNA3.1 (accession number: L07949), on the human adenosine A 2A R FRET construct and on the human AT R in pcDNA3.1 (accession number: NM_000685) using the QuikChange system (Stratagene).

    Article Title: An Alternative Splice Variant of Zebrafish Cx52.6 is Expressed in Retinal Horizontal Cells.
    Article Snippet: Retinal horizontal cells (HCs) are inhibitory neurons, which modulate the transmission of light-elicited signals from photoreceptors to bipolar cells in the outer retina.. HCs of the same physiological type are extensively coupled via gap junctions.. In the zebrafish retina, the population of HCs comprises up to four morphologically distinct subtypes.

    Article Title: Photoswitchable TRPC6 channel activators evoke distinct channel kinetics reflecting different gating behaviors
    Article Snippet: HEK293T cells were transfected with 2 μg cDNA coding for mouse TRPC6 (GenBank: NM_013838 ) or with human TRPC3 (GenBank: NM_001130698 ) using Genejuice reagent (Sigma-Aldrich) according to the manufacturer’s instructions. .. The cDNA was in pIRES2-EGFP expression vector (Clontech, Palo Alto, CA). .. Transfected HEK293T cells were seeded onto poly-L-Lysine-coated glass cover slips (diameter 30 mm, thickness 1, Karl Hecht, Sondheim, Germany) 1 h before patch clamp measurements.

    Article Title: AHR Regulates NK Cell Migration via ASB2–Mediated Ubiquitination of Filamin A
    Article Snippet: .. mAsb2 promoter region (-973/-1 from ATG) was PCR cloned (F; 5’-TCAGATAGGCTGGTGAATGATGC-3’, R; 5’-CTCGGCCACCTCTCCTCCAGCTT-3’) with mAsb2 BAC clone (RP23-213c17, bacpac, CA) and subcloned into EcoRI/HindIII of pBV-Luc reporter vector (Addgene, MA). mAhr expression vector was PCR cloned with cDNA clone into EcoRI site of pIRES2-EGFP expression vector (Clontech, CA). hAhR expression vector (pCMV6-hAhR) was purchased from OriGene (MD, USA). .. To test promoter activity, pBV-Luc reporter vector containing Asb2 promoter and mAhr or hAHR expression vectors were co-transfected into HEK-293 cells with Lipofectamin2000 (Invitrogen, CA).

    Article Title: Contribution of Intracellular Calcium and pH in Ischemic Uncoupling of Cardiac Gap Junction Channels Formed of Connexins 43, 40, and 45: A Critical Function of C-Terminal Domain
    Article Snippet: .. Cx43, Cx45 and Cx40 were sub-cloned into pIRES2-DsRed or pIRES2-eGFP expression vector (Clontech, USA) by using standard molecular biology techniques. .. Cx45 was also sub-cloned into peGFPN1 vector (Clontech, USA) to express it as a fusion protein with eGFP.

    Article Title: Photoswitchable TRPC6 channel activators evoke distinct channel kinetics reflecting different gating behaviors
    Article Snippet: HEK293T cells were transfected with 2 μg 16 cDNA coding for mouse TRPC6 (NM_013838) 37 or with human TRPC3 (NM_001130698) 38 using 17 Genejuice reagent (Sigma-Aldrich) according to the manufacturer’s instructions. .. The cDNA was in 18 pIRES2-EGFP expression vector (Clontech, Palo Alto, CA). .. Transfected HEK293T cells were seeded onto 19 poly-L-Lysine-coated glass cover slips (diameter 30 mm, thickness 1, Karl Hecht, Sondheim, Germany) 20 1 hour before patch clamp measurements.

    Plasmid Preparation:

    Article Title: A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
    Article Snippet: .. After 48 h, the cells have reached a confluence of approximately 80 to 90% and are ready for transfection. c. Transiently transfect the cells with 1 to 2 μg μL −1 cDNA coding for hTRPC6 (NP_004612) in pIRES2-EGFP expression vector (Clontech) using lipofection with GeneJuice® (Merck Millipore) according to the manufacturer’s instructions. d. 24 to 48 h after transfection, and 1–2 h prior to the loading with PhoDAG or OptoDArG, split the transfected HEK293T cells from one well plate onto 3 to 5 glass-bottom dishes (WPI, FluoroDish FD35-100, diameter of glass bottom 23 mm). .. For equal distribution and separation of the transfected HEK293T cells, wash the cells once with 1 mL Dulbecco’s phosphate buffered saline modified without calcium chloride and magnesium chloride (DPBS, D8537, Sigma-Aldrich) and detach the cells by applying 300 μL Trypsin-EDTA solution (T3924, Sigma-Aldrich) for 20–30 s followed by application of 1.5 mL culture medium.

    Article Title: AHR Regulates NK Cell Migration via ASB2–Mediated Ubiquitination of Filamin A
    Article Snippet: .. mAsb2 promoter region (-973/-1 from ATG) was PCR cloned (F; 5’-TCAGATAGGCTGGTGAATGATGC-3’, R; 5’-CTCGGCCACCTCTCCTCCAGCTT-3’) with mAsb2 BAC clone (RP23-213c17, bacpac, CA) and subcloned into EcoRI/HindIII of pBV-Luc reporter vector (Addgene, MA). mAhr expression vector was PCR cloned with cDNA clone into EcoRI site of pIRES2-EGFP expression vector (Clontech, CA). hAhR expression vector (pCMV6-hAhR) was purchased from OriGene (MD, USA). .. To test promoter activity, pBV-Luc reporter vector containing Asb2 promoter and mAhr or hAHR expression vectors were co-transfected into HEK-293 cells with Lipofectamin2000 (Invitrogen, CA).

    Polymerase Chain Reaction:

    Article Title: An Alternative Splice Variant of Zebrafish Cx52.6 is Expressed in Retinal Horizontal Cells.
    Article Snippet: Retinal horizontal cells (HCs) are inhibitory neurons, which modulate the transmission of light-elicited signals from photoreceptors to bipolar cells in the outer retina.. HCs of the same physiological type are extensively coupled via gap junctions.. In the zebrafish retina, the population of HCs comprises up to four morphologically distinct subtypes.

    Article Title: AHR Regulates NK Cell Migration via ASB2–Mediated Ubiquitination of Filamin A
    Article Snippet: .. mAsb2 promoter region (-973/-1 from ATG) was PCR cloned (F; 5’-TCAGATAGGCTGGTGAATGATGC-3’, R; 5’-CTCGGCCACCTCTCCTCCAGCTT-3’) with mAsb2 BAC clone (RP23-213c17, bacpac, CA) and subcloned into EcoRI/HindIII of pBV-Luc reporter vector (Addgene, MA). mAhr expression vector was PCR cloned with cDNA clone into EcoRI site of pIRES2-EGFP expression vector (Clontech, CA). hAhR expression vector (pCMV6-hAhR) was purchased from OriGene (MD, USA). .. To test promoter activity, pBV-Luc reporter vector containing Asb2 promoter and mAhr or hAHR expression vectors were co-transfected into HEK-293 cells with Lipofectamin2000 (Invitrogen, CA).

    Purification:

    Article Title: An Alternative Splice Variant of Zebrafish Cx52.6 is Expressed in Retinal Horizontal Cells.
    Article Snippet: Retinal horizontal cells (HCs) are inhibitory neurons, which modulate the transmission of light-elicited signals from photoreceptors to bipolar cells in the outer retina.. HCs of the same physiological type are extensively coupled via gap junctions.. In the zebrafish retina, the population of HCs comprises up to four morphologically distinct subtypes.

    Clone Assay:

    Article Title: AHR Regulates NK Cell Migration via ASB2–Mediated Ubiquitination of Filamin A
    Article Snippet: .. mAsb2 promoter region (-973/-1 from ATG) was PCR cloned (F; 5’-TCAGATAGGCTGGTGAATGATGC-3’, R; 5’-CTCGGCCACCTCTCCTCCAGCTT-3’) with mAsb2 BAC clone (RP23-213c17, bacpac, CA) and subcloned into EcoRI/HindIII of pBV-Luc reporter vector (Addgene, MA). mAhr expression vector was PCR cloned with cDNA clone into EcoRI site of pIRES2-EGFP expression vector (Clontech, CA). hAhR expression vector (pCMV6-hAhR) was purchased from OriGene (MD, USA). .. To test promoter activity, pBV-Luc reporter vector containing Asb2 promoter and mAhr or hAHR expression vectors were co-transfected into HEK-293 cells with Lipofectamin2000 (Invitrogen, CA).

    BAC Assay:

    Article Title: AHR Regulates NK Cell Migration via ASB2–Mediated Ubiquitination of Filamin A
    Article Snippet: .. mAsb2 promoter region (-973/-1 from ATG) was PCR cloned (F; 5’-TCAGATAGGCTGGTGAATGATGC-3’, R; 5’-CTCGGCCACCTCTCCTCCAGCTT-3’) with mAsb2 BAC clone (RP23-213c17, bacpac, CA) and subcloned into EcoRI/HindIII of pBV-Luc reporter vector (Addgene, MA). mAhr expression vector was PCR cloned with cDNA clone into EcoRI site of pIRES2-EGFP expression vector (Clontech, CA). hAhR expression vector (pCMV6-hAhR) was purchased from OriGene (MD, USA). .. To test promoter activity, pBV-Luc reporter vector containing Asb2 promoter and mAhr or hAHR expression vectors were co-transfected into HEK-293 cells with Lipofectamin2000 (Invitrogen, CA).



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    Image Search Results


    Journal: iScience

    Article Title: Photoswitchable TRPC6 channel activators evoke distinct channel kinetics reflecting different gating behaviors

    doi: 10.1016/j.isci.2024.111008

    Figure Lengend Snippet:

    Article Snippet: The cDNA was in pIRES2-EGFP expression vector (Clontech, Palo Alto, CA).

    Techniques: Recombinant, Expressing, Plasmid Preparation, Software, Inverted Microscopy, Microscopy, Control, Spectrophotometry